FastQCFastQC Report
Thu 28 Apr 2016
ERR1041680.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041680.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751725
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT307970.5354393681895432No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC302930.5266767795748233No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246670.42886264555415987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT218240.3794339958881901No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA200900.3492865183923084No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG187680.32630210936718984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT185180.32195558723687245No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC122460.21291004003146882No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG112460.1955239515101991No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT108160.18804793344605314No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG107360.18665704636435157No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG97520.16954913525942217No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA92630.1610473379725213No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84530.1469646062702928No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA77160.13415105903011706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.12425837466151458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15700.015.554145
TATAACG7550.015.4370872
ACGGACC15700.014.493638
CGCAAGA16350.014.370032
GACGGAC16250.014.0030777
GTACTAG2405.456968E-1213.8750011
GTATAAC10200.013.7843131
AGTACCG6450.013.7674425
CGGACCA16900.013.6834339
GTATTAG19200.013.6822921
TAACGGC4600.013.67391336
ACCGTTA950.001246134813.6315798
TTAACGG4750.013.63157835
ATAACGC19650.013.5572513
TATACTG7700.013.4545455
TCGTTTA10350.013.40579730
GCGCAAG18200.013.2142851
ATAATAC9050.013.0828723
AGACGGA18050.013.0166216
TAATACT12850.012.813234
Produced by FastQC (version 0.11.5)