FastQCFastQC Report
Thu 28 Apr 2016
ERR1041679.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041679.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401047
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG132070.5500517066096582No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC124640.5191068729600046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82670.34430812891209545No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA81520.3395185516984882No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG76280.3176947390034431No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT58530.24376865592385324No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55870.23269015558629214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55000.2290667363029545No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53960.22473529256195318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45780.1906668215990774No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44080.18358657702244063No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC41320.17209159170978328No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC39040.16259573427758808No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC33720.14043873360246592No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT33130.13798147224939789No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.13514937441874317No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA31570.13148430663789587No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC29870.12440406206125912No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA27650.11515809561412167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT400.00193179723.12528
TATACCG951.6780177E-719.4736845
TAATACT2150.016.3488374
ACGGACC5450.016.2935778
ACAACAC11050.016.2398193
CTAGTAC855.3671724E-415.2352943
CGAACGT3200.015.031254
ATAATAC1508.112329E-814.83
TTAGAGT2006.184564E-1114.7999994
TCTTATA10500.014.79999937
GACGGAC6050.014.6776867
ATTAGAC1152.2117154E-514.4782613
TCTAGCG4600.014.47826128
CGTAAAC1152.2117154E-514.47826132
TAAGACT2059.276846E-1114.4390244
GTCTTAA1803.3360266E-914.3888881
GTCGGGA4000.014.3374992
CGAGCCG6600.014.29545415
TCGAACG3400.014.1470593
CAAGACG7200.014.1319454
Produced by FastQC (version 0.11.5)