FastQCFastQC Report
Thu 28 Apr 2016
ERR1041678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6039274
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT638381.057047585521041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT376530.6234689798806943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306160.5069483517389674No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207210.34310415457222176No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT78880.1306117258465173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1851.8189894E-1217.05
TAGAGTG9250.016.65
AAGACGG19500.016.317955
CGAACGT6400.015.6093764
TTAGAGT10100.015.5693074
GACGGAC19400.015.2577327
ACGGACC19800.014.6691928
CGCAAGA20850.014.6402872
TCGTTTA13500.014.38888830
TCGAACG6850.014.31386853
CAAGACG22350.014.2371374
TATACTG7600.014.1184225
ATTAGAG10750.014.1116293
GCGCAAG22550.013.9467851
AGACGGA21250.013.9294126
CACTCTA14050.013.8256239
TCTTATA36650.013.52796737
TAGACTA4650.013.5268815
CGGACCA21800.013.4931199
TATCTAG14150.013.4664311
Produced by FastQC (version 0.11.5)