FastQCFastQC Report
Thu 28 Apr 2016
ERR1041677.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041677.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15416700
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275580.17875420809900952No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC268720.17430448799029624No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG234660.1522115627858102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211410.13713051431240147No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT187280.12147865626236484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182490.11837163595321958No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA161970.10506139446184981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG51150.015.6246335
GACGGAC49500.015.3232327
TCGTTTA33600.015.1413730
ACGGACC50800.015.0767738
CGCAAGA55250.014.5321262
TCTAGCG29950.014.3305528
CGGACCA55700.013.98294459
CAAGACG59200.013.843754
GCGCAAG59400.013.7971381
AGACGGA56850.013.7651716
TATACTG23750.013.6315795
CGAACGA23750.013.55368516
CTAGCGG32100.013.37071729
CGAGCCG46750.013.01925215
TAACGCC41300.012.9903154
CGTTTAT39500.012.97341731
CGGTCCA34700.012.95533110
CGCATCG41400.012.91425113
TATACCG5600.012.8839285
ATAACGC45150.012.7840543
Produced by FastQC (version 0.11.5)