FastQCFastQC Report
Thu 28 Apr 2016
ERR1041676.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041676.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11040015
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1727421.5646899030481392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT931530.8437760274782236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT863910.7825261106982192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC644870.5841205831694976No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG614740.5568289535838493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592860.5370101399318751No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA421250.3815665105527483No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG352070.3189035522143765No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT283420.25672066568750135No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG278580.25233661367307925No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG259640.23518083988110522No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC209950.19017184306361903No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT201900.1828801863040947No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA185450.1679798442302841No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC170410.15435667433422873No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA161520.14630414904327577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT159930.14486393360878586No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT157780.14291647248667688No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC146520.13271721098205028No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.1313404012585128No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC131140.11878607048994044No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC119550.10828789634796691No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG119060.10784405637130022No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA118330.10718282538565392No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114840.10402159779674211No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113910.10317920763694614No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT112100.1015397171108916No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA111940.10139478977157186No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC111140.10067015307497318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30350.016.1532145
GACGGAC30450.015.1888347
ACGGACC31050.015.0740738
CGCAAGA32600.014.6978542
TCGTTTA21550.014.42227530
CGGACCA33200.014.3765069
AGACGGA33450.014.04783256
CACTCTA23550.013.9044599
CAAGACG37500.013.8626664
TCTTATA72850.013.81468737
TTAACGG5650.013.75221335
CGAGCCG25050.013.736526515
ACTCTAA24200.013.45454510
TCTAGCG16850.013.3946628
GCGCAAG36350.013.1815681
TTATCTA17150.013.0524784
TAATACT14200.012.7676064
GTAAACG23000.012.70869527
CTAGCGG17950.012.67688129
ATTAGAG20150.012.6699743
Produced by FastQC (version 0.11.5)