FastQCFastQC Report
Thu 28 Apr 2016
ERR1041675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13604414
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC323170.23754790173248186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275980.2028606303806985No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG270740.19900893930455218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192480.14148349204897764No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA188590.13862412596382323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169620.12468012220151489No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT149090.10958943178294928No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG143990.10584064848364655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG7750.015.75483835
AAGACGG39250.015.6955425
TAACGGC7950.015.12578636
ACGGACC40450.014.6353538
GACGGAC40100.014.6246887
CGAACGA17500.014.37714316
GTATTAG40850.013.9033051
AGACGGA43500.013.779316
TCGTTTA28900.013.69896130
ATTAGAG29200.013.6849323
CGCAAGA43650.013.6471932
CAAGACG46600.013.5375534
CGAGCCG37200.013.37768715
TATACTG21150.013.2955085
ATAACGC36000.013.2069443
TAACGCC32150.013.1197524
GCGCAAG47900.012.8225461
CGGACCA46500.012.7311829
ACGAACG19750.012.55189815
GTAAACG31550.012.54833627
Produced by FastQC (version 0.11.5)