FastQCFastQC Report
Thu 28 Apr 2016
ERR1041673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405701
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC178590.17162707250573508No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG175000.16817704064339348No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA108180.10396224146744175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC30400.014.6661197
ATTAGAG15050.014.5049843
TCGTTTA19700.014.27411230
ACGGACC31550.014.1901748
GTTCAAA35700.014.0952381
AAGACGG35450.014.0380815
CGAGCCG32500.013.94615415
TAGAGTG14150.013.7279145
CGCAAGA34100.013.6173022
TACCGCA31900.013.57053228
CAAGACG37400.013.3556154
CGAACGA13850.013.22382616
TATACTG11200.013.2142855
TCTAGCG18650.013.19302928
AGACGGA34350.013.0873366
TTAGAGT14050.013.0355864
TATTAGA11250.012.9911122
ATACCGC32950.012.9696527
GTATTAA8650.012.832371
CGGACCA35500.012.7676059
Produced by FastQC (version 0.11.5)