FastQCFastQC Report
Thu 28 Apr 2016
ERR1041671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823931
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64640.22890077696657604No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT57330.20301487536345614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43870.15535082124881947No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41870.14826849522881402No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA40870.1447273322188113No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG38490.13629936425500483No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC34490.12213471221499393No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG29560.1046767785756805No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29400.10411019249408006No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG28490.10088773415497759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1002.8786053E-718.55
CAAGACG14250.017.9157894
AAGACGG13950.017.770615
CGCAAGA14850.016.5690232
TATACTG3800.016.5526315
CGAACGT4050.016.4444454
GACGGAC14650.016.4163847
ACGGACC14650.016.2901048
GCGCAAG15450.015.9255661
GGTTATC5800.015.6293112
TCGAACG4400.015.5568183
GCAAGAC17850.015.5462193
GTATACT1802.0190782E-1015.4166664
TTAGACT2055.456968E-1215.3414634
AGACGGA15500.015.2774186
CGGACCA15650.015.1309919
GTATACG750.004106375414.81
TCGTTTA7550.014.70198730
TAATACG908.2792784E-414.3888884
TACTCCG3500.014.2714295
Produced by FastQC (version 0.11.5)