FastQCFastQC Report
Thu 28 Apr 2016
ERR1041670.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041670.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600990
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT30010.49934275112730664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12290.20449591507346213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.1708847069002812No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG7260.12080067887984824No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT7050.11730644436679478No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.10432785903259621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC551.9007002E-523.5454543
TCGCGTA2100.022.904769
GCGTAAC2300.022.5217411
GCTATAC502.7000238E-422.23
CGTAACT2350.022.04255512
CGCGTAA2200.021.86363610
AGACGGA5900.021.0084726
GACGGAC5850.020.8717967
CGGACCA5900.020.6949149
GTCGCGT2350.020.4680868
CAAGACG6150.020.4552844
AAGACGG6250.020.4245
ACGGACC5950.020.2100858
CGCAAGA6050.019.8760322
CGAAAGC6150.019.85365919
GCGCAAG6400.019.656251
GCAAGAC6600.019.3409083
GCGAAAG6350.019.22834618
GTAACTA2550.018.86274513
AGCGAAA6600.018.78030217
Produced by FastQC (version 0.11.5)