FastQCFastQC Report
Thu 28 Apr 2016
ERR1041669.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041669.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526976
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT141480.13439757058437293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA23950.015.91231630
AAGACGG38750.015.7548395
GACGGAC37650.015.5272247
ACGGACC38850.015.1428588
CAAGACG41900.014.9236294
CGAACGA14950.014.84949816
AGACGGA40750.014.6638046
TACCGTC23050.014.3665947
CGCAAGA41250.014.3515152
TAATACT19150.014.104444
CGGACCA42700.014.0807979
GCGCAAG43900.013.7801831
TAGACTA6550.013.5572515
CGTTTAT28350.013.507936531
ATTAGAG21150.013.4704493
TAACGCC29950.013.46577554
ACCGTCG23650.013.2198738
TATCTAG27050.013.1312381
ATAACGC31600.013.1139243
ATACCGT27650.013.1139246
Produced by FastQC (version 0.11.5)