FastQCFastQC Report
Thu 28 Apr 2016
ERR1041668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026641
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT27400.26688978912784506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.11893154471718935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11150.10860661126917784No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG10860.10578186532585393No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA10820.10539224519574028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC7050.022.0425518
GACGGAC7150.021.4755237
TCGTTCG2250.021.37777737
CGCGTAA4350.021.26436610
CAAGACG8000.020.5812514
TCGCGTA4500.020.5555559
AAGACGG8100.020.327165
AGACGGA7550.020.0927166
GTATACA1202.382876E-1020.0416664
GCGTAAC4450.019.95505511
CGGACCA7750.019.8129049
GTCGCGT4850.019.0721638
CGCAAGA8150.019.0674842
GCGCAAG8250.019.0606061
GTAACTA4750.018.69473613
AGCGAAA8150.018.61349717
TAGACTC609.2359015E-418.55
GCGAAAG8200.018.518
CGTAGAC1103.8504368E-818.53
AGACCGC902.151759E-618.56
Produced by FastQC (version 0.11.5)