FastQCFastQC Report
Thu 28 Apr 2016
ERR1041667.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041667.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7753682
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT117830.1519665108783156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30700.017.6563515
ACGGACC31150.016.1540938
GACGGAC30750.016.1235797
GGTTATC14250.016.0982462
AGACGGA32800.015.7926826
TCGTTTA18650.015.57372630
CAAGACG35750.014.9034974
CGCAAGA33750.014.85481552
CGGACCA34750.014.5870519
CGAACGA11450.014.21834116
TTATCTA15400.014.1753244
TAACGCC21150.014.1702134
GCGCAAG36150.014.1244811
ATAACGC23400.013.7564113
TACCGTC19800.013.2676777
CGCATCG22500.013.23777813
CGTTTAT21750.013.18390831
ATTAGAG16700.013.1826343
TAGAGTG14050.013.167265
AGGTTAT17750.013.1323951
Produced by FastQC (version 0.11.5)