FastQCFastQC Report
Thu 28 Apr 2016
ERR1041666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775791
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT31320.4037169804754115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.1988937742252746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15270.196831363086192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10030.12928739828123811No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG8130.1047962660046327No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT7980.1028627555617428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC251.2332559E-436.9999965
ATTAGAG1054.0017767E-1122.9047643
TCCGGTC603.724277E-521.5833328
TTAGAGT1403.6379788E-1219.8214284
CGGACCA7250.018.882769
ACGGACC7350.018.625858
GACGGAC7350.018.625857
GTTCTAC609.2331273E-418.51
TATGACT701.2185492E-418.54
ACTACAC902.150382E-618.53
GCTATAC500.00703287218.4999983
GCGTTGT500.00703287218.49999834
CGCAAGA7450.018.375842
CAAGACG8100.018.2716054
CGAAAGC7450.018.12751619
CGAACGA2450.018.1224516
CGAGACT2450.018.1224520
AGACGGA7700.018.019486
AAGACGG8300.017.6084355
GCGCAAG8000.017.5749991
Produced by FastQC (version 0.11.5)