FastQCFastQC Report
Thu 28 Apr 2016
ERR1041665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980346
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT42060.21238712830990142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA2000.022.231
CGCAAGA10050.018.03982
AAGACGG10550.017.8862555
CGAACGA2700.017.81481416
TAGCACT2300.017.6956524
GTAGCAC1502.5102054E-1017.2666663
ACGGACC10500.017.2666668
CGGCGTT2600.017.07692330
ACCGTCG3800.017.0394748
GACGGAC10550.016.8341227
TACCGTC3850.016.8181827
CGAACGT2250.016.4444454
TTAGACT1801.0913936E-1116.4444454
TACGCTG904.44857E-516.4444455
AGACGGA11050.016.2398196
GCGCAAG11650.016.1974261
CAAGACG12200.015.9221324
CGGACCA11300.015.880539
CGTCGTA3900.015.65384710
TCTACGC957.063795E-515.5789483
Produced by FastQC (version 0.11.5)