FastQCFastQC Report
Thu 28 Apr 2016
ERR1041664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4786029
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84260.17605409411434825No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG71110.14857828901579995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC66490.13892519247167118No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT59150.12358888757255755No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50100.10467968330321442No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG49050.10248579772500334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG14950.017.0769235
ACGGACC15200.016.3092128
TAGAGTG7500.015.7866685
GACGGAC15400.015.6168827
CGCAAGA16500.015.4727282
TCGTTTA9900.015.32323330
CGGACCA16850.015.1513379
GCGCAAG17350.015.141211
CAAGACG17500.014.9057154
TTAGAGT7400.014.54
TACCGTC12000.014.4916677
ACCGTCG12200.014.2540988
CGCATCG12500.014.0613
AGACGGA17550.014.0199436
AATAACG12550.013.8565732
TAACGCC12750.013.6392154
ATAACGC13700.013.6386873
TAGACCG950.001246081413.6315795
ATTAGAG9100.013.6208783
CCGTCGT12700.013.5472439
Produced by FastQC (version 0.11.5)