FastQCFastQC Report
Thu 28 Apr 2016
ERR1041663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3512906
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT321560.9153675048521082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT236200.6723777977549072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174360.49634120582788155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.23732488145142513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.024.6443611
CGCGATA705.1068528E-621.14285914
GTATCAA115950.017.6144892
TGCGACG1352.2235326E-816.44444522
GTACTAG2200.015.9772731
GTAATCG1201.9381623E-615.41666620
TATATCG2450.015.1020415
GTCGGTA1353.9786028E-715.07407524
CTAGTAC2950.015.0508473
TATAGCG1752.242814E-914.82
TACACGC1001.0943809E-414.7999995
GTATAGG8350.014.6227561
TCTATAC5100.014.5098043
CCGTCGT1956.7484507E-1014.230779
GTGTAAG7950.013.9622631
GTACGTA2800.013.87500113
TCGCTAA1753.572677E-813.74285814
CGTATTA2700.013.70370415
ATGTACG2850.013.63157811
TGCGCGA1102.458616E-413.45454512
Produced by FastQC (version 0.11.5)