FastQCFastQC Report
Thu 28 Apr 2016
ERR1041662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7988550
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT454930.5694775647645693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339980.42558411726783957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321910.40296424257218144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130830.16377189852977073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119050.024.506091
GTATCAA185200.015.7629592
CTAGCGG7550.015.19205429
TCTAGCG7550.014.94702128
CTAGTAC5700.014.2807023
GTATAGG16100.014.0186341
TTAACGG5100.013.78431335
GTATTAG21550.013.4779581
TAGACAG13750.013.4545465
GACGGAC9800.013.4030627
TATACTG15850.013.3059935
TAACGGC5150.013.29126236
CGCAATA9750.013.28205136
GTACTAG5250.013.0380951
CTAACGC2305.7661964E-1012.8695653
CGCTATA3600.012.8472222
ACGGACC11000.012.7818178
TATACCG5700.012.6578946
TAATACT21100.012.5379154
TCGCTAA4000.012.48749914
Produced by FastQC (version 0.11.5)