FastQCFastQC Report
Thu 28 Apr 2016
ERR1041661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7303435
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT470930.6448061768195377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT343620.4704909402219641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT317150.43424772042196585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137340.18804850046587668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126600.022.8838861
ACGGACC6800.016.5955878
GTATCAA186100.015.5475562
TATACTG14200.015.37323955
TGCGACG3500.015.32857122
TCTAACG2800.014.5357142
GTATAGG11850.013.8945141
CTAACGC2551.8189894E-1213.7843153
CGGACCA9000.013.77222259
GACGGAC8250.013.4545467
GTATTAG14200.013.158451
TAACGCC6700.012.9776124
CGCGTTA1000.001834113712.95000123
CCGTATA3600.012.8472222
CGTATAC4050.012.7901233
TAGTACT11500.012.7086954
TCTATAC9050.012.6740343
GTACTAG7300.012.6712331
CTAGTAC8200.012.4085373
GTATACG4800.012.3333341
Produced by FastQC (version 0.11.5)