FastQCFastQC Report
Thu 28 Apr 2016
ERR1041660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7956696
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT381020.4788671076537296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278330.349805999877336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257370.32346340742438817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114630.14406733649243356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118000.021.0084741
TATACTG17250.015.5507265
CTCGCTA5050.015.01980113
TGCGACG4050.014.61728422
GTATCAA170300.014.5023492
TCTAGCG4850.014.11340228
ACGGACC7400.014.08
GACGGAC7850.013.9044597
TCGCTAA5200.013.87514
GTATTAG22050.013.4240371
CTAGCGG5250.013.39047729
CCTATAC16650.012.6666673
CTAGTAC7400.012.53
CTAATAC19050.012.4304463
CGTTAAC2102.8987415E-812.3333331
GTATAGG15500.012.2935481
TTAACGG5150.012.21359335
CGGACCA9000.012.1277779
TTTAGCG5350.012.10280426
CGCACTA5200.012.09615429
Produced by FastQC (version 0.11.5)