FastQCFastQC Report
Thu 28 Apr 2016
ERR1041659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714323
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT959631.24395880234727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT691400.8962549273604437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT643350.8339681913759639No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266210.34508536912441956No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA129130.16738993168940425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90330.11709387849069841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA298200.020.6527521
GTACTAG5600.015.8571421
TCTAACG2700.015.7592592
CTAACGC2650.015.3584893
CGTAGCG4600.015.28260915
TAACCGG3650.015.20548122
TGCGACG3350.014.91044722
GTATCAA425750.014.4697592
AATCGTC6300.014.38888928
GTACGTA5500.014.12727413
TCGTAGC5000.014.06000114
TATACTG12250.014.0448985
TCTATAC9650.013.9948193
TTAACGG4950.013.82828335
CGCTATA5900.013.796612
ACGGACC5900.013.796618
TTATACG5800.013.71551735
CTAGCGG3950.013.58227829
TCGCTAA6850.013.5036514
GTATTAG22550.013.4545451
Produced by FastQC (version 0.11.5)