FastQCFastQC Report
Thu 28 Apr 2016
ERR1041658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601144
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT641850.5093585153855872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT449790.3569437822470722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT424550.3369138548055637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184210.14618514001585886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168600.024.8202861
GTATCAA253750.016.4112322
TCTAGCG11150.015.26457428
CTAGCGG11550.015.05627629
TATACTG21250.014.103535
ACGGACC15900.013.9622658
GACGGAC15950.013.9184947
GTATAGG24600.013.4613821
TTAACGG8800.013.45454535
TAATACT31950.012.854464
GTATTAG34400.012.8531991
ATCAACG325150.012.8074734
TAACGGC9250.012.836
TAACGCC12750.012.6235294
CGCTATA7650.012.5751632
TCAACGC330900.012.5401945
AACGCAG332750.012.5260717
CTAATCG8500.012.40588326
CAACGCA335000.012.3977616
CGTATTA7500.012.33333415
Produced by FastQC (version 0.11.5)