FastQCFastQC Report
Thu 28 Apr 2016
ERR1041657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6784646
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT796911.174578599974118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT559680.8249214476333768No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT515100.7592142611419962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218270.3217117002125092No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.12818649639200042No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72460.10679997158289467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202900.023.8977321
TGCGACG3300.017.93939622
TATACTG7900.016.6265815
GTATCAA299800.016.1612762
TTAACGG6300.015.56349235
GTACTAG5250.015.1523821
TAACGGC6600.014.85606236
CGTTCGG1152.2128928E-514.47826124
ACGGACC4650.014.322588
CTAACGC2500.014.0599993
CGCGAAT2252.7284841E-1113.977777535
TCTATAC7150.013.9720283
GTACGTA6050.013.76033113
GTATTAG22350.013.5749441
TTTAGCG4650.013.52688126
CTAGTAC6250.013.323
GACGGAC5050.013.1881197
TTATACG5900.013.16949235
CGTAGCG4500.013.15555615
CAATGCG4500.013.15555619
Produced by FastQC (version 0.11.5)