FastQCFastQC Report
Thu 28 Apr 2016
ERR1041656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5840234
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250280.4285444727043471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176280.30183722090587467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157430.269561116900453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69980.11982396595752842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.026.8266181
GTATCAA91500.016.5792352
TCTATAC6950.014.6402873
CGAACGA2950.013.7966116
GTATAGA8750.013.5314281
GTCGCTA2202.8558134E-1013.45454537
ATCAACG118500.012.7860764
TCAACGC120400.012.59966855
CAACGCA121150.012.5827496
GACGGAC5600.012.2232147
AACGCAG125300.012.2102967
TATCAAC126250.012.1770313
TATACTG11400.012.1710535
CTAGTAC3650.012.1643843
ACGGACC5200.012.0961548
TTAACGG2451.5661499E-912.08163435
CTACACT14400.011.9479164
GTAAACG3750.011.84000127
TACTCCG7050.011.5460995
TAATACT14750.011.5389834
Produced by FastQC (version 0.11.5)