FastQCFastQC Report
Thu 28 Apr 2016
ERR1041655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890086
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146070.772821977412668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111850.5917720146067428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG81000.42855192832495453No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC77400.40950517595495656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69450.36744359780454433No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG64530.34141303623221375No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA63450.33569901052121437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.16147413398120508No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29350.15528393946095573No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28540.1509984201777062No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG27640.1462367320852067No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC24010.1270312567787921No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20380.10782578147237745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG852.72462E-517.4117648
GTTATAC1502.5102054E-1017.2666663
TCTACAC2200.016.8181823
TACACTG4350.015.3103465
TACCCCG1353.9757833E-715.0740755
CGTATAC750.00410570714.83
CGCAAGA2950.014.4237292
TATACTG4000.014.3375015
AGAGCGC1304.44732E-614.23076910
TCTATAC1601.785047E-713.8753
TACTCCG2551.8189894E-1213.7843135
CGTATCT950.00124559313.6315799
TACACCG1805.163747E-813.3611125
CGCGGGT1254.8476104E-513.3222
ACGTTTA1409.565814E-613.21428626
CGATGCG2102.042725E-913.21428533
CCGTATA1000.001833240212.9500012
GTCGGTA1000.001833240212.95000124
GTAGCAC2152.903107E-912.9069773
TAAGACT2305.7661964E-1012.8695654
Produced by FastQC (version 0.11.5)