FastQCFastQC Report
Thu 28 Apr 2016
ERR1041654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6435692
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239480.3721122763488371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176370.2740497836130132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157270.24437154543753803No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65320.1014964668912061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.024.6135061
GTATCAA104100.016.3318922
TTAACGG4250.013.92941235
TAGACCG800.006302774413.8755
GTATAGG12900.013.7674411
ATACCGA3150.013.5079376
TATACTG12100.013.4545465
TCTAGCG4150.013.37349428
GTATTAG16750.013.1432851
ATCAACG132250.012.88355354
AACGCAG134450.012.75537
TCAACGC134850.012.6763075
CAACGCA135050.012.6164386
TAACGGC4750.012.46315836
GTCGCTA3150.012.33333437
TACGGGT1501.946705E-512.3333334
CTAGTAC5100.012.3333333
GTATACG3001.8189894E-1212.3333331
CTACACT20900.012.1267944
TAATACT18550.011.9676554
Produced by FastQC (version 0.11.5)