FastQCFastQC Report
Thu 28 Apr 2016
ERR1041653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940830
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48710.2509750982826935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33850.17440991740647044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30610.1577160287093666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA200.001842248137.014
CGACGAG2100.020.26190624
GAACCGT555.144849E-420.1818186
ATCGGTC555.144849E-420.18181826
TGCGACG1950.019.92307722
GGTATCA22300.019.8273561
CTCGCTA2250.019.73333413
TCGCTAA2350.018.89361614
CGTAGCG801.6176053E-518.515
GCGCTAG1103.855712E-818.511
TCGGTCC701.2197467E-418.527
CAATGCG2150.018.06976719
TACGCAG650.001580463617.0769235
TAGGTCG2400.016.95833421
GTCGTCA2400.016.95833424
TTAACGG2000.016.65000235
GTACTAG3000.016.651
CGCACTA3450.016.62318829
CATTGCG2450.016.61224429
TGATCGC1452.9831426E-916.5862069
Produced by FastQC (version 0.11.5)