FastQCFastQC Report
Thu 28 Apr 2016
ERR1041652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551380
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107021.9409481664188037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72911.3223185461931881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38140.6917189597011135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31760.5760092857920128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.18335811962711743No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.14654140520149445No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.14436504769850195No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7660.1389241539410207No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.11516558453335268No Hit
CCTGTAATCCCAGCTCTCAGGGAGGCTAAGAGGCGGGAGGATA5540.10047517138815337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.033.825741
CGTAGCG451.07824235E-732.8888915
GTATCAA24900.025.4839362
GAATCGG601.3352983E-624.66666822
GCGTTAT451.3219923E-424.66666811
TCGTAGC601.3352983E-624.66666814
TCCGGTC851.9717845E-923.9411778
TTAACAC400.001929919623.1250023
GTGGTAT1200.023.1250021
GCGGAAT652.6786438E-622.7692319
ACACCGC753.7334576E-722.26
ACCTGAT502.6996317E-422.225
TCACGTA1005.329639E-1022.225
CCGGTCT1107.4578566E-1121.8636369
CGTAGGA1059.786163E-1021.14285728
AGCGTTA450.003823320820.55555710
TCTATAT450.003823320820.5555573
CTCCGGT1001.2862074E-820.357
ACTCCGG1001.2862074E-820.356
CGGTCTG1101.7480488E-920.18181810
Produced by FastQC (version 0.11.5)