FastQCFastQC Report
Thu 28 Apr 2016
ERR1041651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2150921
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52910.24598764901174894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38140.17731939015891332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30480.14170673864823485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.022.2542781
CGCGATA656.9057045E-519.92307714
TATATCG904.448856E-516.4444455
CGGATCG1403.477362E-815.85714337
TACCGTC2055.456968E-1215.3414647
CGTCGTA1752.240995E-914.810
GTATCAA30750.014.6796742
TATACCG1406.0013554E-714.5357155
TAACACT4250.014.3647064
TATACTG3100.014.3225815
TACTCCG2201.8189894E-1114.2954545
CAGTACT5050.013.9207924
CTACGTA800.006300974613.8759
TAGGACC3500.013.7428584
TCTAGAC2304.0017767E-1113.6739123
GTACTAG1805.1650204E-813.3611111
ATAACGC1951.0271833E-813.2820523
CGCTATA1551.8894953E-613.1290322
ATACCGA1551.8894953E-613.1290326
CGTACGG1703.735313E-713.05882422
Produced by FastQC (version 0.11.5)