FastQCFastQC Report
Thu 28 Apr 2016
ERR1041650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4860218
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1573833.2381880812753665No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1185772.4397465298881658No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA999552.056594992241089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT854581.7583161907552294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT822711.6927430004168538No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG681081.4013363186589574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT623911.2837078501417014No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC599521.2335249159605597No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG538511.1079955672770234No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT486781.0015600123286652No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG470700.9684750766323651No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC412030.8477603268001559No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA407310.838048828262436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367020.7551513121427887No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT306750.6311445289079626No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG266400.5481235615357172No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT249330.5130016801715479No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC247980.5102240269880898No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC233780.48100723053986466No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC219640.45191388534423765No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG210330.4327583659827604No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA207010.4259273966723303No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC193270.39765705982735755No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT189650.39020883425393676No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC186840.38442720059059077No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC164500.33846218420655205No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG160220.32965599485455177No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG158650.32642568707823394No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT157430.32391551160873855No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA152850.3144920659937476No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG151940.31261972199600924No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT137110.28210668739550365No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT136970.28181863447277467No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT133220.27410293118539125No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132580.2727861178243445No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA131490.2705434200688117No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC130110.2677040412590546No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT126510.26029696610316655No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT124670.2565111276901571No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT121340.24965958317096065No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT116860.24044188964363328No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG113860.23426932701372652No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC109130.2245372532672403No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT104700.2154224357837447No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG103270.2124801809301558No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100230.20622531746518363No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT98790.20326248740282846No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94770.19499125347875343No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94450.19433284679823004No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG85880.17669989288546314No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC84470.17379878844940702No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG74300.1528738011340232No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT73400.15102203234505118No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA68500.14094018004953687No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC66250.13631075807710683No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA66030.135858103484247No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA60980.12546762305723735No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT60360.12419196011372328No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT60100.12365700468579804No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.12338952697183543No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG59730.12289572196144288No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG57740.11880125541693809No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG57100.11748444205589131No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.11672315933153615No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA56080.11538577076172303No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT54480.1120937373591061No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT54310.11174395881007808No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT53940.11098267608572289No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.10894573041785369No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT51400.10575657305906855No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA51010.10495413991718067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG10850.024.211985
TATAACG12600.023.4920642
TATCACG11150.022.7309422
ATAACGC14100.022.5673753
GTATAAC13950.021.8817211
CGTTATA1550.021.4838712
ATCACGC12600.020.408733
AAAAGTA24200.019.723142
GTCTAAC1551.8189894E-1219.0967751
TAGCGGA1950.018.97435817
TAACGCA16700.018.9431134
TACAACG4200.018.9404752
TCTAACG609.242667E-418.52
AACGAGT701.2202254E-418.56
AAAAAGT25950.018.250481
GTATCAC14150.018.0424021
CGTAGCG1950.018.0256415
GTACTCC30750.017.98861736
TCACGCA14400.017.9861134
TGGGCTG27000.017.12963136
Produced by FastQC (version 0.11.5)