FastQCFastQC Report
Thu 28 Apr 2016
ERR1041649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8726642
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2482322.8445305765952127No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1782162.04220592525739No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1613301.8487065242277616No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1034071.1849575128669194No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC949081.087566099308302No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG798500.9150140454942464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT792260.9078635287204403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT766050.8778290664381558No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT757090.8675616577373061No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG675800.7744101339323878No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC659100.7552733342332595No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA633140.7255253509883871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT601310.6890508399450785No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT487540.5586799595995802No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG452700.5187562409458301No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC413830.47421448020899676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401060.45958113097798675No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT393900.4513763713465042No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC390090.44701043081634384No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC363210.4162082047137948No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG332900.3814754862179519No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA329800.3779231461540418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC322290.36931731587018235No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT308270.3532515714521118No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC291660.3342179042064519No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC290950.3334043037402016No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG265110.30379383043328695No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT260360.29835072872245705No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG255620.2929190861731236No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG247860.28402677685185207No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT221860.25423295696099374No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA219820.251895288015711No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT201590.23100523660762065No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC199580.22870194514682737No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT184910.21189135523148536No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT183790.21060792914387919No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG181590.20808691361465267No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC177220.20307926004068919No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG174700.20019155134357522No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT173800.19916022680889167No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT172120.19723508767748235No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT171560.19659337463367926No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT168240.19278893301684658No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161730.1853290188826355No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA148660.17035189480673094No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG148490.17015708906129068No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA147270.16875907135871965No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC134950.15464138439505137No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG131260.1504129538028488No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129330.14820133563402738No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT117380.134507637645729No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117170.13426699525430286No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA113950.130577145252435No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC111250.12748317164838432No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA110210.12629141885275No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT105620.12103166372586385No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT101370.11616152008985815No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG100520.11518749136265702No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT99100.1135602904301563No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA96090.11011108281971463No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG91980.1054013674446597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14800.022.3752
CACGCAG13600.021.6286775
GTATAAC17500.020.8257141
TATCACG14600.019.7671222
CGTAGCG3750.018.25333215
ATAACGC23200.018.1810343
CGTTATA2600.017.7884622
TAACGCA20300.017.7709374
AAAAGTA49700.017.494972
ATCACGC16650.017.3333343
TCACGCA17450.016.9627514
AAAAAGT51450.016.7560731
GTATCAC18700.016.4224591
CTAACGC1151.2444161E-616.0869563
TCTAGCG9050.015.74033228
GTACTCC48350.015.68769536
GCGGAAT4650.015.5161319
GTCTAAC2150.015.4883721
CCGCCTA1455.358561E-815.31034515
TTAGCGA3800.015.09210627
Produced by FastQC (version 0.11.5)