FastQCFastQC Report
Thu 28 Apr 2016
ERR1041648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8017334
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1321531.6483409572309198No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG987271.2314193221836585No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA923821.1522783009913269No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT729950.9104647505018502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT665870.8305379319359776No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG635180.792258374167772No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC502320.6265424391699286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT502260.6264676013248294No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG500360.6240977362300236No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT460030.5737942313492241No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG441610.5508190129037907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378450.4720397079627717No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA368430.4595417878312167No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC348340.43448358269719084No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT293130.36562029223180675No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC254000.31681354425298985No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG236480.2949608934840435No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC222020.27692497281515277No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT219800.2741559725464849No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC218150.2720979318062588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC198550.2476509024072092No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG189870.2368243608162015No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT183530.22891649518405993No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC180670.225349224567668No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA173550.21646846694923774No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG165150.20599116863535935No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC160760.20051553296893954No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG156720.19547645139893136No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT148610.18536086933636542No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT143870.17944867957353405No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT138340.17255112485023075No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT135930.1695451380720823No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131510.16403208348311296No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA127590.15914267760330306No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT127160.15860633971342591No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA127050.15846913699741086No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT126630.15794527208171694No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC123260.1537418797819824No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG120580.15039912270088787No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG118430.1477174332515023No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT118300.14755528458712086No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT117380.1464077709622675No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG111040.13849990533012596No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101950.12716197179760755No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT100430.12526607972176287No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT98700.12310825518807124No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC93070.11608597072293608No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88420.11028603772775339No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC87390.10900132138688497No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG83150.103712780333213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG11500.019.6260872
CACGCAG12750.018.5725485
GTATAAC13950.017.6379931
TATCACG13250.017.5924532
TCACGCA15150.016.118814
ATCACGC14600.016.0924663
TAACGCA15950.015.42633154
ATAACGC21750.014.9701153
CGTAGCG3350.014.91044915
GTACTCC40950.014.86324836
CGTTATA2251.8189894E-1214.82
TATACTG7850.014.6114655
AAGACGG17500.014.1657145
AAAAGTA40750.014.0282212
TATGTCG1203.305197E-513.8750015
GTATCAC18050.013.7340721
TAACGGC2304.0017767E-1113.67391336
TCGTAGC3800.013.63157914
CGAACGA11150.013.43946216
AAAGTAC43300.013.2875293
Produced by FastQC (version 0.11.5)