FastQCFastQC Report
Thu 28 Apr 2016
ERR1041647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5752893
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1965903.4172372056980724No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1446852.5149954987864365No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1258432.187473328636566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT916121.5924509633674744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT911771.5848895503531875No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG831391.4451685438960884No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC712601.2386811296507687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT707391.229624816592278No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG668401.1618502204021524No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT616671.0719302444874257No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG560460.9742228822959161No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA510990.888231364636888No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC507750.8825994156331433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455390.7915843385232438No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT393190.683464823698268No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC327520.5693135610205161No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG324130.5634208736369684No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT314680.5469943557093796No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC300740.5227630689463545No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG266040.4624455904185946No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA261510.4545712913485441No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC257710.44796591906020156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC249520.433729603522958No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT238680.4148869099425281No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC238350.4143132855069615No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG207760.3611400385858037No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG205470.3571594326541446No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC205190.3566727210118457No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT198700.3453914404457027No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT198100.3443484869264907No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG192510.33463163663916573No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT189760.32985143300944414No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA185660.32272458396149556No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT174390.30313444035896375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT172710.3002141705051702No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169940.2953992017581415No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA169070.29388691915528414No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT167850.2917662469995531No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC160790.2794941605901587No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT151510.26336314615968004No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT145270.25251642955987535No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG144550.25126488533682095No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT139840.2430777002110069No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG131370.22835467303146434No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.2235570868430892No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC125540.21822064133645455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124350.21615211685668412No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT122270.21253654465674923No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA118190.20544446072610772No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC109860.19096478936771463No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG102480.1781364610814072No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT96490.1677243084479409No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG94190.16372631995762826No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA90720.1576945721048523No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA88600.1540094696703033No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC86310.15002886373864419No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT83210.14464027055604892No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT79610.13838254944077702No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT79270.1377915424465569No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT79180.13763509941867508No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77360.13447147374373208No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT75600.13141214342071025No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA75020.13040395501880533No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG74590.1296565049967034No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG74130.12885690729864088No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT72430.12590187232754024No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA71520.12432005949006873No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66150.11498562549312147No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT65500.1138557591806418No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT64830.11269112775085509No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA62510.10865837414323543No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG60910.10587716475867011No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58290.10132293439144445No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA58230.10121863903952324No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA58020.10085360530779905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG12150.023.6008245
GCGCATA753.746154E-722.226
GTATAAC15350.021.6938131
TATAACG15000.021.2133352
TATAGCG705.1077877E-621.1428592
TACAACG4700.020.8617022
GAGCGTA759.2763585E-619.7333346
TATCACG14000.019.5571422
ATAACGC17150.019.524783
TAACCGG951.6790364E-719.47368422
ACAACGC5250.019.0285723
CAACGAG1900.018.51
AAAAGTA32500.018.4430772
TGGGCTG36500.017.94246536
AAAAAGT33300.017.8333341
GTACTCC40200.017.6716436
GTACCTG48850.017.64790236
ATCACGC15900.017.5691813
CGAACGA2350.017.31914916
GGGCTGT39250.017.25095637
Produced by FastQC (version 0.11.5)