FastQCFastQC Report
Thu 28 Apr 2016
ERR1041646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7067472
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2402373.399192808970449No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1909522.7018430352465495No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1673912.368470649759914No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1224151.7320903429118646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1178201.6670741673967722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1139931.612924678017826No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG916161.2963051003244157No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC890311.2597290799312684No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT881901.2478294926389522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT795401.1254377802982451No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG793571.1228484527423668No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC686150.9708563401453871No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA647770.9165512081264701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT587960.8319240599750519No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT582750.8245522585727966No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC469080.6637168141592921No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT449530.6360548722372016No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC414330.5862492274465325No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG406060.5745477307869066No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC387830.5487535005444663No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG383320.5423721523056617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC356260.5040840628728348No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA350130.49541052302718713No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT341830.48366657837484184No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC335840.4751911291618842No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG304320.430592438144785No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG301160.42612124957834996No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT280290.3965915959766094No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC277810.3930825619118123No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT267500.3784946017472726No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT245910.3479461963202684No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG243370.3443522662700326No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT243000.34382874102649436No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC239670.33911701383464976No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT237810.3364852382860519No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT237150.3355513824462269No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA235070.33260832161768733No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT225420.3189542172929727No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT219090.3099976908291961No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA218120.3086252057312714No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG211420.2991451540239565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194210.27479415553397313No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT191210.2705493562620411No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG173090.24491076865957162No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC169220.23943497759877932No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC162440.22984173124421292No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160600.22723825435742798No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT156650.22164926864938408No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA155970.22068711414774617No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG146210.20687736718306066No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT134450.1902377540370871No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA131790.186474032015974No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT130690.18491760561626563No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG128050.18118218225696542No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA126400.17884754265740282No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC125090.17699398030865918No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT122380.1731595116330139No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG114620.16217963084961637No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG110820.15680288510516915No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT110080.1557558346180926No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT106600.15083186746265143No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105610.14943108370291386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105600.14941693437200743No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA104890.14841233187765016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT101910.14419583126753102No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT101390.14346006606039613No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT95510.13514025948740935No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA80520.11393041245865566No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80190.11346348453874314No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT79910.11306730327336281No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA79190.11204855144809912No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78120.11053457304111003No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG74080.10481824335490825No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73680.10425227011865064No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.10184688386455581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG12300.022.1097565
TACAACG4300.021.941862
AAAAGTA41200.019.9817962
GTCGTCA1700.019.58823624
AAAAAGT42200.019.2452621
TCTAACG1103.8598955E-818.52
CGCTATA500.00703838218.4999982
CTAACGC1258.600182E-917.7600023
GGGCTGT52850.017.64238437
TATCACG14950.017.5719052
TGGGCTG52850.017.5373736
TATAACG16150.017.5263162
GTACAAC6000.017.2666661
CTCCTGT32350.017.09891737
GTTACGT650.00158102317.07692319
ACAACGC5750.017.0521743
GTATAAC17550.016.8660961
TAGGTCG2200.016.81818221
GCGTTAT3200.016.7656251
GGCGTCG2550.016.6862758
Produced by FastQC (version 0.11.5)