FastQCFastQC Report
Thu 28 Apr 2016
ERR1041645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7909007
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2037522.5762020440745594No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1401201.7716509796994744No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1315761.6636222473946476No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG815401.0309764550720464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT807851.0214303767843422No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC802491.0146532933906873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT776550.9818552442803502No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG688670.8707414217739344No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT619970.7838784312619776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT596190.753811445608785No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG575190.7272594397754357No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC515850.6522310575777718No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA512720.6482735443273726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417490.5278665197792846No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT391690.4952454840411698No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG358650.4534703281967003No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC334230.4225941385562056No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC305460.3862178905645171No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT301580.38131209139149835No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC276160.34917152052084416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC263870.333632275202184No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA262730.33219088059980223No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG259860.3285621064692445No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT247290.31266883440613974No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC239880.30329976949065796No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC229150.2897329588910466No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG219680.27775926864143624No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT210530.266190180385477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT209880.2653683325858733No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG209630.26505223727833344No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG195990.247806077298958No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT194870.24638997032117937No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT181930.2300288772029156No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179720.22723459468426316No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA174350.2204448674783067No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT174210.22026785410608438No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA173740.21967359492790942No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT164490.20797806854893414No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC160680.2031607760620265No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT157040.19855842838424598No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG151540.1916043316183688No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149850.18946752733939926No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT148320.18753302405725522No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG147950.18706520300209623No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC138080.17458576026042208No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT137510.17386506295923115No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA124710.1576809832131897No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122490.15487405688223566No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG115080.14550499196675384No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC108670.13740030828143154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT107440.13584511936833538No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG103740.13116690881674525No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT96210.12164611815364433No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA93850.11866217845046792No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT92740.1172587152849909No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC90460.11437592608022726No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT89910.11368051640363955No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA88860.1123529161119721No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT84300.10658733770244483No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT82710.10457697154649123No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG81760.10337580937783972No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA79180.10011370580402824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG17750.020.5323942
CACGCAG13850.019.9025275
GTATAAC19350.019.5038761
TATCACG14150.019.0883392
GCGAATC2850.018.17543833
CGTTATA2200.017.659092
AAAAGTA42000.017.0464292
TAACGCA23150.016.7818574
ATAACGC28850.016.4159453
GGCGAAT3500.016.38571432
ATCACGC16600.016.382533
AAGACGG15700.016.2611475
AAAAAGT43850.016.1584951
TGGGCTG47750.016.11727736
TCACGCA17950.015.4596094
GTATCAC18350.015.4250691
GTACTCC48900.015.28425436
TTAACGG2201.8189894E-1215.13636435
TACAACG6000.015.1083342
GGGCTGT52350.015.05444137
Produced by FastQC (version 0.11.5)