FastQCFastQC Report
Thu 28 Apr 2016
ERR1041644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7246460
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2538783.5034761800934526No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1878072.591706847205394No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1677232.3145508289564836No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1101471.5200111502719948No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1025681.415422150953707No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG881221.2160696395205384No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT810081.1178975665359363No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC733401.0120803813172226No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG716510.9887724488922868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT705470.9735374237903749No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT694520.9584265972626633No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA646780.8925461535701571No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT558620.7708867502201074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT539590.7446256516975185No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC469000.6472125699996963No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG440900.6084350151660258No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT433430.5981265335073953No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC413090.5700576557381121No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395640.5459769321848185No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA380290.5247941753628668No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC373950.5160450758025298No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG372450.513975099565857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC343490.4740107583564941No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT326920.45114442086204853No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC326400.450426829100002No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT301990.4167414158085465No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG299380.41313965715673584No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT298150.41144227664266414No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC294430.4063087355757156No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG287790.397145640768044No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG282950.3904665174443797No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT263170.3631704308034544No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT242460.3345909588957919No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA237750.3280912335126393No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT231250.31912133648705715No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC226000.31187641965870233No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT215240.2970277901209694No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT211770.2922392450934663No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT202070.2788533987629822No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG201620.27823240589198034No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT197740.2728780673597867No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG187210.25834683417834364No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC176060.2429600108190758No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176020.24290481145276452No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA174660.24102803299818118No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC157900.21789949851375706No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA151180.2086260049734629No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG142200.1962337472365817No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT141040.19463296561355475No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137940.19035501472443095No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA137010.18907162945769385No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG135630.18716725131995485No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC131760.18182671262933903No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT130350.17988093496686658No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT129850.17919094288797566No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA124380.17164242954490883No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT121020.16700568277476174No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120710.16657788768584939No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT117550.16221713774725866No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT108660.14994907858457784No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT108590.1498524796935331No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG108140.1492314868225313No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA106650.14717531042743628No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT106410.14684411422956867No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG105180.14514673371549694No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT92510.12766233443640065No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG78690.10859095337585525No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78530.10837015591061015No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA77090.10638297872340426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG12650.021.7905142
CACGCAG9700.020.4072175
GTATAAC14950.019.799331
AAAAGTA48050.019.0197722
TATCACG10850.018.9262682
AAAAAGT50600.017.9881421
TGGGCTG56200.017.84163736
GGGCTGT57300.017.59598537
GTACTCC68150.017.07483536
TACTCCT69950.016.84703437
ATAACGC19250.016.6259733
CTAACGC904.451195E-516.4444453
CGTTAAT2250.016.4444431
GATACAA12150.016.1399171
TGCGACG700.00259427315.85714322
CTCCTGT33600.015.47172537
TGTACCT80650.015.41475435
GATACAT5800.015.3103461
GTACTAG1455.358197E-815.3103461
GTACCTG83600.015.29126736
Produced by FastQC (version 0.11.5)