FastQCFastQC Report
Thu 28 Apr 2016
ERR1041643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14376345
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4768793.317108764432128No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3567752.481680844470552No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3073372.1377964983450246No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2063241.435163109955973No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1835741.2769170467180635No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1607051.1178432348416791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1566101.0893589434588555No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1506511.0479089086968907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1425770.9917472069569838No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1352170.9405519970479284No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1319480.9178132550380503No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1194830.8311083241254992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1139580.7926771373391499No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1019180.7089284515640102No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC844700.5875624158991732No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG837080.5822620422645672No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT802240.5580277880087046No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT757270.5267472365194352No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC751640.522831081196229No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC701760.4881351970893854No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG700370.48716833103267904No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA683660.4755450707394682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC619050.430603188779902No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT610200.4244472430231745No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC591710.4115858377077067No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG544380.37866370068331No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG539160.3750327360674775No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT534020.37145741841893753No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC533020.37076183132778184No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG532810.37061575803863916No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT516100.35899249774542835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT469300.32643902187934415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT440650.30651045171773494No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA434330.3021143413016313No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT428060.2977530102400854No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC413040.2873052921309276No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT412220.28673491071617996No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT392550.27305271263314845No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT370910.25800020798054024No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG365830.2544666255574696No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT356520.24799070973881054No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC332130.23102534058552435No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328940.22880641776473784No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG327170.22757522861339236No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA313120.21780222998265555No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC294060.20454434002522892No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA275370.19154381729152994No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG261710.18204209762634382No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259590.18056745299309387No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC237430.16515324305308476No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA236950.16481936124933008No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG235450.1637759806125966No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT234250.16294127610320983No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229840.15987373703121344No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA225140.1566044777027819No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT222530.15478899539486565No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT211190.14690103778116065No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT207980.14466820321855103No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT203750.14172586982296265No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG199710.13891569797469383No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT193500.13459610213861728No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT192790.13410223530389678No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA190610.13258585544517748No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG190090.13222415015777655No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT187530.13044344720441808No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT169410.11783940911267782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG23500.020.4680845
TATCACG23750.020.0189482
TATAACG24800.019.8427412
AAAAGTA86450.018.7674962
GTATAAC28500.018.6947361
AAAAAGT89900.017.9032271
TGGGCTG105300.017.65669436
ATCACGC27900.017.3064523
CGTTATA4500.017.2666662
ATAACGC38950.017.1463413
GGGCTGT110350.017.10013637
GTACTCC110700.016.8622436
GTACCTG148950.016.3202436
TACTCCT115900.016.31320237
TCACGCA30900.016.1051774
TGTACCT148000.016.02535
TACCTGT159350.015.998116537
AAGACGG19250.015.9532475
TAACGCA33400.015.8967074
CTCCTGT60050.015.18817637
Produced by FastQC (version 0.11.5)