FastQCFastQC Report
Thu 28 Apr 2016
ERR1041642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4997738
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2024104.050032234582925No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1468852.93902961699873No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1297812.596794789962979No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG837531.6758181401265935No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC797021.5947614700890684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT688641.377903363481639No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG677661.3559334242811447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT676301.353212193196202No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT621561.243682642027253No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG578221.1569634102467956No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC542971.0864315016113288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT542341.0851709313293334No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA525511.051495696653166No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT396790.7939391780841653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346940.6941940533897535No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC339210.6787270561201888No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC311770.6238222171710481No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT309900.6200805244292518No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG297350.5949691640498161No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG275870.5519897201493956No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA273140.5465272489274148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC260260.5207555898288385No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC246390.4930030345728408No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT242310.48483934131801226No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC235470.47115314968491745No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT225890.4519844777777467No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG222520.4452414272216751No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT221350.44290036812654043No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG216910.43401634899628594No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT211420.42303137939603874No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG205180.41054573088865404No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT195470.391116941304246No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC185510.3711879254174588No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT184010.3681865676031837No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT183520.36720612405052044No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA175710.35157905436419434No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC167120.3343912786144452No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA159970.3200848063664002No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG150510.3011562430843714No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT149110.29835497579104786No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG148010.2961539800605794No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142310.2847488203663337No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT141010.2821476435939619No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT131720.26355923419755095No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125870.2518539387218778No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA122800.24571115972866125No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC115560.2312246060117597No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC112780.22566208952930306No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG105680.21145566254173387No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103350.2067935534035598No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT99870.19983040327444138No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT97200.19448798636503153No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA96790.19366761522912965No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG93970.18802506253829232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT93640.1873647638191518No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT93620.18732474571496147No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA92170.1844234331611621No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC91370.1828227089935487No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT87230.1745389614261492No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT86830.17373859934234248No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT81590.16325385604447454No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG78440.15695100463449663No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT69200.13846264049856155No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG68080.13622162666390275No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA67370.1348009839651458No Hit
GTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTGTGTTGTG65660.13137943605687213No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.12625711872050915No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT60900.12185512725957223No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58460.11697291854835126No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA57210.1144717870364553No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA55770.11159048353475112No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA54670.10938948780428266No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG53040.1061280123127703No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCT52430.10490746013496505No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51750.10354684459249364No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA51120.10228627431049808No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT51020.10208618378954638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1450.028.0689662
ACAACCG358.8735705E-426.428575
CACGCAG10450.025.846895
CGTACCC400.001932087223.1253
TACAACG2500.022.9399992
AAAAGTA32950.021.8406662
TATAACG15050.021.6345522
AAAAAGT33400.021.380241
GTACAAC2950.021.3220331
TATCACG12450.020.9518052
GTATAAC15850.020.7760241
ATAACGC16500.020.7424243
GCGTTAT2750.020.1818181
GGGCTGT39550.020.02022737
TGGGCTG39500.019.95189936
GATACAT4500.019.7333341
GTTATAC2550.019.5882363
CTCCTGT21450.018.9743637
GACTGTC7150.018.62937237
ATCACGC14550.018.4364243
Produced by FastQC (version 0.11.5)