FastQCFastQC Report
Thu 28 Apr 2016
ERR1041641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4975470
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1085052.1807989998934776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG726291.4597414917585676No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA674671.3559924992010803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT549751.104920741156112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT529021.063256335582367No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC473790.9522517470711309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426710.8576275206161428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG417170.8384534526386452No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG411610.8272786289536467No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT351550.7065664148311617No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG338310.679955863466165No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA307270.6175697974261728No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC286590.576005884871178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276460.5556459992724305No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT214370.4308537685886961No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT206840.415719519964948No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC181180.36414650274245447No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC166570.33478244266370816No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA145850.2931381356937134No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG143860.2891385135474639No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT141340.2840736654024645No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG137500.2763558015624655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC137490.27633570295871546No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC136370.27408465933871573No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT135340.272014503152466No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT124160.2495442641599688No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT121790.2447808950712194No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG121650.24449951461871944No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA117380.23591741081747053No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG114040.22920447716497133No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113250.22761668746872155No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110790.22267243094622216No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT107150.21535653918122308No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC104600.21023139522497372No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG104070.20916616922622389No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT100530.20205126349872476No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT97100.19515744241247562No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC94070.18906756547622636No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA93780.18848470596747643No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87350.17556130375622805No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC85330.17150138579872856No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT82940.16669781950247917No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79390.15956281517123005No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75290.15132238763373107No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG73660.1480463152224815No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71680.144066791679982No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT71040.14278048103998214No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG70150.14099170530623237No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT66750.13415818003123323No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT60710.12201862336623476No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT58080.11673269057998542No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA58020.11661209895748542No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC57270.11510470367623561No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC56780.11411987209248572No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT56140.1128335614524859No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.11204971590623598No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC54310.10915551696623635No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT53750.1080299951562365No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA52340.10519609202748685No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT50810.10212100565373723No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT50210.10091508942873739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG10650.022.9295772
TATAACG14200.022.7992972
GTATAAC15250.021.7147541
CACGCAG12250.021.4448995
GTATCGT450.003827571620.5555554
ATAACGC17800.020.162923
ATCACGC12650.020.0355743
TAACGCA16700.019.8293424
TCACGCA13900.018.899284
ACGTCGA500.007038092718.528
AAAAGTA30450.018.2873572
TACAACG4150.018.277112
GTATCAC14000.018.103571
CGTTATA1351.1550583E-917.8148142
CGTCCTA953.6112706E-617.52631614
ACAACGC4350.017.4367833
AAAAAGT32750.017.0595421
GTACTCC32050.016.85491636
TACGCAG1606.311893E-1016.18755
TAGCGGA1751.3278623E-1015.85714217
Produced by FastQC (version 0.11.5)