FastQCFastQC Report
Thu 28 Apr 2016
ERR1041640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7365430
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2335253.170554875954289No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1563662.122971774899768No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1478102.0068074776353857No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1004711.3640887225864615No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG880541.1955038606028434No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG760961.033150814005428No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT693240.9412077774142176No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG655720.890267099137457No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC623720.846820891651947No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA595510.8085203443655021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT485930.6597442376073087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT470490.6387814424955501No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT436050.59202246168927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT386770.5251153021615846No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC382180.5188834867753818No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC362140.49167529933758114No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT358990.4873985632882262No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG341890.4641819961631568No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG303220.4116799698048858No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA300800.40839435036379407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC292470.39708475947772226No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC280360.3806430853324246No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT278530.378158505341847No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT265120.35995182901745043No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG259620.3524845121058784No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC259410.35219939636925474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256260.3479226603198999No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT247230.3356626836450825No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG244830.33240421808366927No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG232270.3153515816456066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT226780.3078978416738738No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT210950.2864055459083856No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC208420.2829705801290624No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG204700.27791995850887186No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT202860.275421801578455No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA198060.2689048704556285No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT189660.25750024099068214No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC182950.24839011435856426No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG169310.22987116841786562No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT165340.22448112330169454No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT154900.2103067981095469No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT145900.1980875522542472No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG140550.19082388944026352No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA139090.1888416562237371No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA133520.18127930073329052No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC131090.1779801043523596No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121410.16483762658799284No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC120850.1640773179569964No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117250.15918961961487652No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA113350.15389461307757998No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT109400.14853172184108734No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG109300.14839595244269513No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC108090.1467531427221493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT103590.14064351979449943No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA101820.13824040144295718No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT95750.12999919896054948No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT94920.12887231295389406No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT93560.1270258491357599No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT87720.11909691626965431No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG83270.11305517804120059No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT78200.10617166954271509No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG78060.10598159238496598No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77390.10507193741573811No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA76100.1033205121764785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14900.023.093962
GTATAAC16800.021.4732131
CACGCAG11650.020.484985
TATCACG11350.020.3744492
AAAAGTA49850.018.8525582
TACAACG5300.018.8490542
TAACGCA19550.018.6419434
ATAACGC25050.018.6107793
AAAAAGT50850.018.263521
TTAACGG2000.017.57499935
GGGCTGT55250.017.3782837
ATCACGC13550.017.2029513
TGGGCTG54850.017.13436
TAACGTG1301.3966201E-817.0769235
TCACGCA14900.016.7617454
GTACTCC53650.016.4827636
TAACGGC1951.8189894E-1216.12820636
AAGACGG17300.015.9335275
TACTCCT55900.015.720036537
TACCTGT72150.015.58974437
Produced by FastQC (version 0.11.5)