FastQCFastQC Report
Thu 28 Apr 2016
ERR1041639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505907
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4054543.859295537262989No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2792282.6578190726417055No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2603492.478120166112264No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1812591.7253055828497244No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1624481.5462539312407773No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1346821.2819645176756276No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1182691.125738120468799No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1073081.0214063383580303No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1050000.9994377448800946No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA936460.8913652100670603No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT764110.7273146430860277No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG743570.7077637371052303No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC670790.6384884237029702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT636100.6054689043030744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT629960.5996245731092041No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT595920.5672237532656628No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC574040.5463973743533043No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC566780.5394869762315619No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA521480.4963683763810207No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG500060.47597984638546675No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC483510.46022680383521386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC479150.4560767575802832No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC474190.45135560404256386No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT473710.4508987182163329No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG462760.4404760103054406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT461270.43905776055318213No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT460200.4380392858988757No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT429610.4089223329313689No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG407930.3882863231132733No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG403670.3842314614054741No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT391700.372837871113841No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT357280.3400753499912002No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT348610.33182284975490456No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA343020.3265020335702572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337520.3212668834780281No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT326580.3108536940218488No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC323840.30824563743044747No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT317940.30262974914969265No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC300090.28563930748673105No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG299270.28485879420025323No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG291020.27700606906190967No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT273360.26019647803849777No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT259680.2471752319909171No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG239850.22830013629475304No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA230740.21962882405107909No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG194810.18542901626675354No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC194430.18506731498765408No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT189770.1806317150913291No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA183320.17449231180135136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180160.17148448011199796No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC177860.16929523552797487No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT176260.16777228277387188No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176150.1676675797720273No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA175020.16659199438944206No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT172030.16374597643021208No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG169980.1617946932140176No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT166930.15889156452650874No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA165490.15752090704781604No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT165220.15726390877056118No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA161930.15413233716993688No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT157460.1498775879131616No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT140430.1336676595366778No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG139700.1329728123426183No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT134640.12815647425776755No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG119860.1140881981917411No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA119820.11405012437288851No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA117240.1115943630568974No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC111870.10648295287593922No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT108540.10331330745646235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA3150.024.6666662
TATAACG17350.023.0317022
GCGAATC3650.022.8082233
GTATAAC19550.022.427111
TATCACG13350.020.9250952
CACGCAG14850.020.3063965
AAAAAGT73700.020.1567171
AAAAGTA74600.019.9383372
ATCTCGT4000.019.88749937
TAACGCA22000.018.9204564
GGCGAAT4550.018.70329732
ATAACGC29300.017.994883
ATCACGC16150.017.984523
GTTATAC5650.017.6814163
TGGGCTG82450.017.63614336
AAGACGG19500.017.5512815
GGGCTGT83300.017.36734837
TCACGCA17700.017.3502834
CTCCTGT45800.016.64192237
GACTAAG5200.016.36538526
Produced by FastQC (version 0.11.5)