FastQCFastQC Report
Thu 28 Apr 2016
ERR1041638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979992
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2461942.74158373415032No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1623551.8079637487427604No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1620811.8049125210801968No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1065421.1864375825724567No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG929001.0345220797524095No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG822540.9159696356076932No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT736750.8204350293407834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT715510.7967824470222244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT706980.7872835521457036No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG677080.7539873086746626No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC644460.7176621092758212No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA623940.6948113094087388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT559100.622606345306321No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT473540.5273278639891884No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC411280.4579959536712282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398630.4439090814334802No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT385470.4292542799592695No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC380820.4240761016268166No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG365580.40710503973722917No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA315850.35172637124843764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC309150.3442653401027529No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG308370.3433967424469866No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT302960.3373722381935307No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC284000.31625863363798096No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG276040.3073944832022122No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC275700.3070158637112371No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT275040.3062808964640503No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT265720.29590226806438136No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG260030.2895659595242401No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG259160.28859713906203927No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT246580.2745882178959625No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC236430.26328531250361914No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT220150.24515612040634333No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT216300.24086881146442No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA213620.2378843990061461No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT204210.22740554780004257No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG198460.22100242405561163No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188500.20991110014351907No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC186990.20822958416889462No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185830.20693782355262674No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG183680.2045436120655787No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA172750.19237210901746907No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT169540.18879749558796935No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT157980.1759244328948177No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT152000.16926518420060954No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA148700.16559034796467526No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC145640.16218277254589983No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG141270.1573163985001323No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC124420.1385524619621042No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT121770.13560145710597515No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120760.13447673450043163No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG119740.1333408760275065No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT117120.1304232787735223No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA116940.13022283316065314No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC113590.12649231758781077No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT110750.12332973125143096No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT107600.11982193302622096No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT107250.11943217766786429No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA103780.11556803168644247No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT101040.11251680402387886No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT96190.10711590834379361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG15650.021.987225
TATAACG18750.021.4106662
TAACGGC5200.020.99038536
TATCACG16350.020.9327242
GTATAAC19950.020.6791971
TTAACGG5200.020.63461535
ATCACGC18550.018.8490563
GTATCAC19700.018.2182731
TCACGCA19850.018.0806054
TAACGCA23050.017.9783084
ATAACGC31050.017.576493
AAAAGTA55350.017.313462
AAAAAGT55800.017.0743731
GTACTCC60050.016.54371336
TGTACCT79700.015.76097935
GTACCTG82150.015.51612936
TGGGCTG62150.015.50844836
AAGACGG19600.015.102045
GTACGTA2950.015.05084713
TATTAGA12550.015.0358562
Produced by FastQC (version 0.11.5)