FastQCFastQC Report
Thu 28 Apr 2016
ERR1041637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12243084
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6984785.70508215086983No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4826013.9418254420209813No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4463073.6453805266712216No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC3223152.6326291643510737No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2742032.2396562826817163No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2190591.7892468923679687No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1959201.6002503944267636No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1863671.5222226687328126No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1790531.4624828188714543No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1593791.301788013542993No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG1321571.079442075215689No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1285701.050143901650924No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1118580.9136423469772812No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1053190.8602326015242565No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1003070.8192952037248131No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC1000510.8172042273008989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT915830.7480386477786153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT886220.7238535650004525No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA874200.7140357772600433No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG867880.7088736792135054No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT838300.6847131000653104No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC828630.6768147633390411No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG815100.6657636262235888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC814050.6649059991747177No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC779800.6369310216282107No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT752600.6147143971241233No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT748880.6116759470081231No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG716170.5849588224666269No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT704190.5751737062328413No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG701470.5729520437824326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT661600.5403867195553016No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT624270.5098960359987729No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA596450.4871730031420188No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT587590.4799362644248786No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT563230.46003931689107086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT546300.4462111017126077No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC542450.44306646920008064No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC535310.4372346052677577No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT532080.43459638110789733No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG512720.4187833719020469No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG475960.3887582573149053No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT452080.3692533678605815No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT438070.35781017266564535No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG419860.3429364692752251No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA397180.3244117250196111No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC342410.27967626457516753No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT330290.26977679806819915No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG327130.26719574904493015No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA302960.2474539911675849No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300120.24513431419730516No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC298960.24418684050521913No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT297140.24270028695384266No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT293090.23939229690819733No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA292000.23850199835270264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT290950.23764437130383162No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT290420.23721147384106814No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG283540.231591974701799No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277740.2268546062413686No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT271470.22173334757811022No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA265660.21698781124102393No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT242410.19799749801602276No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG240000.19602903974194735No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT229160.18717506144693608No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG215670.17615659583810747No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA208460.17026755676919314No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA199010.16254891332935395No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA194240.15865283616448275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC181800.14849199760452514No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA168810.13788192582849224No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT167590.13688544487647067No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160990.1314946462835671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA153510.12538507454494308No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA153410.12530339577838395No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG149850.12239563168887839No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG145190.11858940116722225No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC144960.1184015400041362No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG140840.11503637482189945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC135730.11086258985072717No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT126080.10298058887776967No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCT124720.10186975765256533No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC123010.10047305074440394No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC122950.10042404348446846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG451.3242321E-424.6666665
CACGCAG16800.023.345245
CGTTATA3450.023.0579722
AAAAAGT99300.022.8967781
TATAACG23700.022.7151892
AAAAGTA102750.022.2180042
GTATAAC24800.022.0060481
GGGCTGT128850.021.65153137
TGGGCTG129550.021.43458236
TATCACG19000.020.7394732
CTCCTGT57800.020.48442837
ATAACGC29250.020.4290583
CAACGAG3200.020.2343751
TACAACG5500.018.8363632
GACTGTC26750.018.74205637
ATCACGC21350.018.6299763
ACTATCG500.007038666418.58
GCGGAAT2000.018.519
CGTCCTA1501.2732926E-1118.514
TAACGCA30450.018.22664
Produced by FastQC (version 0.11.5)