FastQCFastQC Report
Thu 28 Apr 2016
ERR1041636.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041636.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4654792
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1034442.222311974412605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT949462.0397474258785357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT786461.6895706618040078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552261.1864332498637962No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC314920.6765501014868119No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA218730.4699028442087208No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205100.4406211920962312No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA203910.43806468688611644No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG175780.37763234103693566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG175410.3768374612657236No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168920.3628948404139218No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT141950.30495455006367633No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133540.28688714769639545No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC132960.2856411199469278No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA124690.267874482898484No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT123050.26435123202067895No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG119300.2562950181232588No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA108010.23204044348275926No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG101530.2181193058680173No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97700.20989122607411886No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT83210.17876201557448754No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81640.17538914735610098No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77550.1666025033986481No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC77120.16567872420507726No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT69090.1484276848460683No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA68980.14819136923841064No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67580.14518371605004046No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66730.14335764089995856No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC66470.1427990767364041No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.14224051257284964No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63550.13652597151494633No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC62150.13351831832657615No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61310.13171372641355403No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA60680.13036028247878745No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA58890.12651478304508557No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA57330.12316339806375881No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA56010.12032761077186693No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA55760.11979052984537224No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA55240.11867340151826332No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG53900.11579464775225187No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAA51130.10984379108669089No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA50770.10907039455253853No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA46550.10000446851330845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG12050.022.8755195
TATAACG16100.022.0621132
GTATAAC17900.020.773741
ATAACGC22650.018.214133
TAACGCA21300.017.892024
TATCACG15900.017.5691812
TCTAGCG6050.016.81818228
AAGACGG8100.016.4444455
TATATCG1708.54925E-1116.323535
CTAGCGG6450.016.06201629
CGTTCGG700.002594085915.85714324
ATCACGC17700.015.7824853
TATACTG4000.015.7249995
TTAGACT2650.015.3584894
AATCGTC855.368515E-415.23529528
AAAAGTA18250.015.0027412
GTACAAC6300.014.9761911
TTACACG750.00410690614.8000014
TCACGCA19550.014.7621494
TACAACG5900.014.7372882
Produced by FastQC (version 0.11.5)