FastQCFastQC Report
Thu 28 Apr 2016
ERR1041635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008446
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1205043.006252298272198No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG945772.3594430360294236No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA779841.9454920934446915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT716601.7877252182017669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT653451.6301828688723758No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG539961.3470556919065393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT484171.2078745728394495No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC457081.1402922728658438No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG417861.0424488692126574No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT372680.9297368606188035No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG355220.8861788333932901No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337060.8408744935069601No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC316590.789807321839935No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA297490.7421579335233655No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT244270.6093882766538454No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG211460.5275361075089947No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC200140.4992957370512164No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT190690.47572051613019106No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC182860.4561867616527702No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC171020.42664913036124225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT163110.40691579729401367No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG160990.40162696466411174No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA154110.3844632059406563No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT148750.3710914404235457No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT148370.3701434421219595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC147410.367748499044268No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC146770.36615187032580704No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG141950.3541272602898979No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA140260.3499111625802119No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC134680.3359905559411303No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127430.3179037462398146No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT126120.31463564683171485No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG124990.31181659925068217No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT122640.3059539781750833No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT119650.2984947283810235No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG111100.27716476659533396No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT104580.26089911152601286No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102810.25648343522651923No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT102510.2557350155147406No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA99810.2489992381087334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.23580210385770445No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG92860.2316608481191963No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT91950.22939064166013465No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC86090.2147715099567264No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT86020.2145968786906447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85930.2143723527771111No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT81390.20304626780552862No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT70230.17520505452736546No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69770.17405747763597165No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG69590.1736084258089045No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG68160.17004095851609327No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC67360.16804517261801705No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT64000.15966287184609695No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT62010.1546983544246324No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT59280.14788773504744732No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT55020.13726017514019148No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55000.1372102804927396No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA52830.13179671124420786No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA51750.12910240028180497No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA51010.12725629832608448No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC50110.12501103919074874No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49390.12321483188248014No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48980.1221919916097161No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT48940.12209220231481227No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA48460.12089473077596656No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG47910.11952262797103916No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG46780.11670358039000651No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA46120.11505705702409363No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT43520.10857075285534594No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT41840.1043796024693859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCA1100.030.27272824
TAACCGG1100.026.9090922
CGTGCGG451.3240144E-424.66666810
CACGCAG6500.023.0538465
TAGGTCG1450.022.96551721
CTGTGCG1550.022.677429
TATAACG8800.022.284092
GTATAAC9250.021.5999981
CGCCAGC1600.020.812534
CGTAGCG2050.020.756115
CGGACCA450.003827443320.5555579
AACCGGC1450.020.41379423
GATACAA5200.020.2788471
GCCGAGT1750.020.08571412
TTCGGTA759.275205E-619.73333426
CGGTAAG759.275205E-619.73333428
CGGCTTT1600.019.6562526
CGAATTC2450.019.63265214
GGTCGTC1700.019.58823623
CGTTCGG851.24689E-619.58823624
Produced by FastQC (version 0.11.5)