FastQCFastQC Report
Thu 28 Apr 2016
ERR1041634.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041634.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6987104
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC924141.3226366746508997No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG670740.9599685363206273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT616270.88201062986897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT590310.8448564669997756No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA578450.8278823386627707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT469020.6712652337792596No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG374840.536474052769216No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC350250.5012806450283265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312890.4478107095586383No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG311010.4451200382876797No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT271000.3878574012924382No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG265450.3799141962106189No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA236660.338709714353758No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC234680.3358759222705144No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT174080.24914471002578467No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC146150.20917106715457506No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT138040.1975639692782589No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG132650.18984975749609567No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC132180.189177089678356No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124200.1777560488580104No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT116330.16649244093117835No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA114540.16393057839127628No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG114380.16370158509162022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC111450.15950814529166876No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA111280.15926483991078422No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT108490.1552717692480318No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT107220.1534541349320119No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC106900.15299614833269978No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC104190.1491175743197754No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG96290.13781103014925783No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94260.13490567765987166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93740.1341614494359895No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG92860.13290198628788122No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT90250.12916653308724185No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT87220.12482997247500538No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG84960.12159544211736366No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT84140.12042185145662637No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC80980.11589923378841935No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79640.11398141490379993No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT77990.11161992150109687No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA75530.10809914951888508No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT73840.10568040779126804No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC72360.10356221976944954No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT69900.10004144778723775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7550.020.337752
TAACGGC5000.019.9836
TTAACGG5200.019.21153835
GTATAAC10750.017.2093031
AAGACGG20150.016.250625
ATAACGC20400.015.325983
TAACGCA10150.015.3103454
GACGGAC20850.015.1726627
TATCACG10600.014.8349052
CACGCAG11850.014.83122355
ACGGACC21300.014.7652588
TATACTG7300.014.698635
CGAGCCG23350.014.65738815
ATAATAC9500.014.6052633
CGCTATA1956.7484507E-1014.230772
AGACGGA22650.014.1302436
TCTAGCG13250.014.10188828
CGCAAGA22700.014.0991192
CGGACCA22400.014.0401789
TCACGCA12150.014.008234
Produced by FastQC (version 0.11.5)