FastQCFastQC Report
Thu 28 Apr 2016
ERR1041633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612138
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1639712.4798484242161916No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1157151.7500390947678346No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1042211.5762072721410232No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG651580.9854301286512774No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC643320.9729379513857696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT639860.9677051507394431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT639440.9670699552852647No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG544190.8230167004983864No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT477410.722020623284027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT475430.7190261304286147No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG446240.6748800463632186No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC416310.6296148084023655No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA391600.5922441425148719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300580.4545882133736471No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT300390.4543008630491378No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG269290.40726615203735916No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC251730.3807089325721877No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT244970.3704853105001741No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC230130.348041737785872No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC201030.30403176703208556No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA198900.30081041865732383No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG198340.299963491385086No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT190730.28845435470342573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC189890.28718396379506905No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC180500.2729828082837957No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG169500.2563467368648386No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC167220.25289853297072745No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT162510.24577526966315585No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT158170.2392115833033128No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG156910.2373059969407777No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG153490.23213369109961104No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT144930.21918780279540445No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT135190.20445731773898246No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA134920.20404897780415351No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT132730.20073688722165206No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT127600.19297842845990207No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA126840.19182902716186503No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC123490.18676258722972813No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117970.17841430411766965No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT112600.1702928765249606No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG112180.16965768107078225No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT110790.16755548659147768No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC108900.16469710704767504No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG102140.15447348497566143No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT98490.14895333400482566No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA94250.14254088465788223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93290.14108900933404597No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG84020.12706933823825212No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83050.12560233921312594No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC76550.11577193337465128No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.11537871714111231No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG72430.10954096844318736No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT69350.10488286844587938No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT66590.10070872688985016No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT66530.10061798468211038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG11300.022.7566382
GTATAAC13400.021.2611941
TAACGCA13400.020.4328374
TATCACG10950.019.9360732
CACGCAG11450.018.5807865
ATAACGC21900.018.2465743
TTAACGG2600.017.78846235
CGTTATA2250.017.2666652
AAGACGG15250.016.9836065
TCACGCA13150.016.7414444
ATCACGC13100.016.6641223
GTACTCC34350.016.26492136
TAACGGC2950.015.67796636
GTATCAC15250.015.649181
TGTACCT46050.015.62757935
TACCTGT50800.015.5137837
TCTAGCG14050.015.01067628
ACGGACC17500.014.9057158
GTTATAC4350.014.88505653
CGGTCCA14950.014.84949810
Produced by FastQC (version 0.11.5)