FastQCFastQC Report
Thu 28 Apr 2016
ERR1041632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9592147
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4325234.509136484251128No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG3006503.134334784485684No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2895053.018145989630893No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1769711.8449571300356429No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1649121.7192397072313423No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1564941.631480418304682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1550641.6165723898935243No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1409631.4695667195258788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1368941.427146602319585No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1176601.226628407592169No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1158281.2075294509143781No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1120591.1682368921160196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1099111.1458435739152038No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT907390.9459717412587609No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT872150.9092333551602159No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC809030.8434295262572602No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT694060.7235710628704919No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC685240.7143760411511625No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA591850.6170151479121411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC569700.5939233416668864No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG569040.5932352788171408No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG554260.5778268410607136No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT546670.5699141182886376No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG534900.5576436641348387No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC502600.5239702852760701No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT456710.4761290668293553No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG449360.4684665487299141No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG448180.46723637575612637No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC426490.4446241284667552No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT418680.4364820514114306No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT389160.4057068766773486No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA388950.40548794758879325No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT384700.4010572398442184No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC382340.39859689389664277No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369520.3852317943000665No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC347020.3617751062405528No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT344720.3593773114611359No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT328530.34249892125297915No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG322260.3359623241803947No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG319710.33330389953364975No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT318860.3324177579847348No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA301920.3147574781745943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297800.31046229796103No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT294800.3073347395530948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259010.2700229677464284No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA255230.26608224415243015No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT251130.26180791432825207No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG237600.2477026259084645No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC224820.23437922709066075No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT221760.2311891175145669No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA218420.22770710248706572No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT216940.2261641736724844No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC215380.22453784330035811No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC210160.2190958916705509No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA200650.20918153151739646No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT197450.20584546921559896No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT185830.1937313929821968No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG178990.18660055981210463No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT176630.18414021386452897No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT175960.18344172582009013No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT167480.17460116072032675No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG161180.1680332880636629No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA148720.15504349547603888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT144170.15030003189067057No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG143470.14957026826215236No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140040.14599442648241318No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT134920.14065672679953717No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133450.13912422317964893No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA123190.1284279734245107No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT111590.11633474758049474No Hit
GTATCACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109790.11445821253573366No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA109600.11426013383656443No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA108230.11283188216360737No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104880.10933944194141312No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCT104220.10865137909166739No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG101800.10612848197593303No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101030.105325741984563No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA100240.10450215160380674No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG100020.10427279732055816No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCT96830.10094716021345378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC3150.032.3015933
GACTAAG3400.031.55882326
CGAATCT3250.031.30769334
AGACTAA3650.029.9041125
GTAGCGA250.00549833129.623
ACTAAGG3650.029.3972627
CCCACGA3650.028.89041119
ATCTCGT2850.027.9122837
GAATCTC3950.026.22784835
CACGCAG19950.026.1503755
CGAGCCC4400.025.64772815
GCGTTAT2800.025.1071411
ACGAGAC4200.025.10714122
AGCGTCA2300.024.9347843
TAAGTTC2300.024.9347843
CGTTATA2700.024.6666682
ATAAGTT2350.024.4042572
CTAAGGC4450.024.11235828
TATAACG28900.022.980972
GCCCACG4850.022.88659918
Produced by FastQC (version 0.11.5)