FastQCFastQC Report
Thu 28 Apr 2016
ERR1041631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512861
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC556542.2147663559584077No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA482741.9210772103988243No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG396801.5790765983474613No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG298041.1860584409563442No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG282251.1232216982952898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT267041.0626930817104487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260131.035194545181767No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT250030.9950013152339108No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG220220.8763715939719706No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC166740.6635464516342129No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA153200.6096636463377799No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152790.60803203997356No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC140100.5575318332370951No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC138670.5518411086009135No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT132040.5254568398331623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130250.5183334852186412No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC119680.4762698772435085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC107950.4295900171159487No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT104260.4149055598379695No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT98960.39381406293463905No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90380.3596697151175493No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79730.3172877449250078No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC78910.31402453219656795No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG76820.30570731926676403No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG75800.30164820099480233No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT73330.2918187675323068No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC71480.2844566412547292No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT71210.2833821687709746No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA67890.270170136748511No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA66030.26276821519375726No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG64440.25644076612275807No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC63270.25178471869315494No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG61660.2453776790678036No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT54990.218834229191348No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT54950.21867504808264362No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC52540.2090843862832047No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA52040.2070946224243999No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT48640.1935642281845275No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG48640.1935642281845275No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47220.187913298825522No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT47110.18747555077658495No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46560.1852868105318997No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT46400.18465008609708217No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46050.18325725139591884No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT43840.17446249514000178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42820.17040337686804005No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40090.15953926619896602No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.15516178570959555No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA38640.15376895100843224No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT35290.1404375331544403No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG34660.1379304306923463No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT33530.13343356437144752No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT33190.13208052494746028No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC33060.13156318634417105No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT31330.12467860339270655No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.10792479170157045No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG27100.10784520114721825No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA26200.10426362620136967No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG25970.10334833482631947No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT25870.10295038205455852No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC25260.10052287014681671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG200.001842351537.01
AGCGCGA250.005497568329.633
CGTAGAC400.001931821523.1253
TCGAATT450.003827053120.55555513
AAAAGTA19050.018.1601052
AAAAAGT19100.018.0157071
CACGCAG3650.017.7397255
TTAACGG852.7251974E-517.41176435
TATAACG5100.017.4117642
CGAACGT1502.5283953E-1017.2666664
CGAATTC752.0684971E-417.26666614
CGTCCTA752.0684971E-417.26666614
CGTTATA650.001580639517.0769232
GTATAAC5750.017.0521741
ACGTCTG1708.54925E-1116.323537
TCGAACG1606.311893E-1016.18753
AAGACGG6400.015.89843755
GAACGTC1659.749783E-1015.696975
TATCACG4250.015.6705872
TACCGTC4250.015.6705877
Produced by FastQC (version 0.11.5)