FastQCFastQC Report
Thu 28 Apr 2016
ERR1041629.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041629.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211217
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1559943.704249864112916No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1036392.461022550013452No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1031962.4505030256099363No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG653071.5507868627999934No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC550271.3066769059870342No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG541631.2861602714844664No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT499821.186877807531647No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG464101.102056721370568No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA378370.8984813653630294No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC348530.8276229887939758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331240.7865659736840919No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT306000.7266308052992757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286110.6793998029548228No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC268440.6374404358645018No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG266530.6329049298575685No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC245950.58403544628548No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT234580.5570361251866147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC215270.5111823969175656No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC210790.5005441419903083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208290.49460761580322266No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT208080.49410894760350754No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG189950.45105725969476285No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG182770.43400755648545297No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC182550.4334851421809895No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA181540.4310867856014069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179580.4264325490707318No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC171000.4060583911966541No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT169280.4019740611799392No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG167520.397794746744231No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT155680.3696793587221936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT150980.35851868949047266No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG149460.35490928156872464No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT148700.35310457760785063No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG144000.34194390837612976No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA136630.32444302917660145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT132220.31397099698258246No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT127420.3025728667033782No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG127200.3020504523989146No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC123200.2925520104995777No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT120680.2865679921029954No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT117240.27839933206956563No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG98790.23458776880887403No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT97810.23226065054353645No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA97180.23076464594439092No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG90880.2158045999529352No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC88990.2113165861554985No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT79740.18935143926328185No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC77630.18434101116138163No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT76960.18275002214324268No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA75890.18020918893517004No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA74100.17595863618521676No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT73550.17465260042405795No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73010.17337031076764745No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT67100.15933636286137712No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA65640.15586943156811914No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG65020.15439717307372192No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT64270.15261621521759625No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT63930.1518088476561526No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA61930.14705962670648415No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC60150.1428328200612792No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT59220.14062443231968336No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG58180.13815483742585574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53090.1260680701089495No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT50690.12036900496934734No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.1144087326775134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46660.11079932475576537No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA45680.10847220649042784No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG44800.10638254927257369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8200.023.9146352
GTATAAC8950.022.5307271
AAAAAGT32900.021.3115481
AAAAGTA33300.021.2222212
CACGCAG6100.019.7131165
TCGCATG851.2469209E-619.58823630
CACCGTA951.6787635E-719.47368431
CTCCTGT18400.018.8016337
AAGACGG9700.018.55
ATAACGC13500.018.53
TATCACG6350.017.7716542
TGGGCTG30200.017.70364436
GTTATAC2300.017.6956523
CGCAAGA10400.017.4326922
TAACGCA11000.017.3227274
CGTTATA1502.5283953E-1017.2666662
CAAGACG11300.017.1902644
GGGCTGT32100.017.00155837
TTAACGG1452.9867806E-916.58620635
GCGCAAG10900.016.4633031
Produced by FastQC (version 0.11.5)