FastQCFastQC Report
Thu 28 Apr 2016
ERR1041627.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041627.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258269
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1054754.670612756939054No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG782093.463227808556022No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA660512.9248508481496223No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG451061.9973705524009762No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC386401.7110450526487322No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG331831.4693997924959339No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT308121.3644078716928763No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG297421.3170264481335041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT265201.1743507970042542No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC253311.1216998506378115No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA235481.042745571940278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221730.9818582285812718No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG221350.9801755238193501No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT212030.9389049754480091No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC176200.7802436290805037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT170470.754870212538896No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC162070.7176735809595757No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT154290.6832224150444434No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC144710.6408005423623138No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT141240.6254347909837137No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG135430.5997071208080171No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC133530.591293596998409No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA131880.5839871157953282No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT131650.582968636597323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC127210.5633075599053966No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT126600.5606063759454698No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125120.5540526837148275No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG123860.5484731889779296No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC123000.5446649624114753No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT108660.48116499850106426No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG105950.4691646566463074No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT105300.46628634586933615No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG102160.45238189073135215No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT98280.4352005894780471No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT95850.4244401353426009No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT88820.3933100972470507No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA86010.3808669383496829No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC86000.38082265664542175No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG80410.35606918396346937No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC78600.34805419549221106No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT73720.32644472381279643No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT70380.31165463458959053No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG68130.301691251130844No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG67240.29775017945160653No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT64910.28743254235877125No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60220.2666644230603174No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC59190.2621034075214246No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT58230.25785236391235944No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57730.25563827869930467No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT55510.24580774035334146No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT51630.22862643910003638No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50600.22406542356114353No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG48510.21481054737057456No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA46530.20604276992687762No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT46220.20467003709478365No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA45850.2030316140371231No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG45630.20205741654337903No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT44570.1973635558917029No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.19700930225761415No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT42880.18987994787157775No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA42470.18806439799687283No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC40130.17770247919977644No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG38530.17061740651800117No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG35370.156624387971495No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT33680.14914077995136982No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.14816658245762573No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA33070.14643959599144302No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.14360556691873289No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC28330.1254500681716837No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTC26970.1194277563921747No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA26840.11885209423678046No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA26670.11809930526434184No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTC26080.11548668471293722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA24930.11039428872291121No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24650.10915440100360055No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA24490.10844589373542302No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC24190.10711744260759017No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG24150.10694031579054576No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG23050.10206932832182525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAG3400.026.6617645
CGTAGCG358.872058E-426.4285715
TCGTAGC358.872058E-426.4285714
TGGTCGT358.872058E-426.4285711
AAAAGTA15950.022.0376172
TGGGCTG25000.021.97836
AAAAAGT15950.021.9216291
GGGCTGT25500.021.69215837
TACAACG1450.021.6896552
TATCACG4150.021.397592
GGTCGTA450.00382693420.55555712
GCGAACA555.1452074E-420.18181829
AAGACGG1850.020.05
TATGCGG759.272269E-619.7333325
GACTGTC4900.019.63265237
CGGGTAA851.246397E-619.58823624
GATAATG851.246397E-619.5882361
TATAACG4750.019.4736842
ATCACGC4650.019.0967733
CTCCTGT12650.019.01185837
Produced by FastQC (version 0.11.5)